Research Group on Endothelial Cells (LINCE)

Cardiovascular Area

The LInCE group was established in 2000 as a basic research group with a translational vocation. Our scientific objectives are mainly focused on studying the vascular effects of sex hormones and their relationship with sex differences in cardiovascular physiology and pathophysiology. To this end, we search for new signalling pathways regulated by sex hormones in experiments performed in patients, experimental animals and cultured cells, mainly endothelial cells.

As a result of its scientific activity, the LInCE group has developed 40 competitive research projects funded by regional, national and international agencies, has published more than 150 articles, and has presented more than 200 communications to national and international scientific congresses.

A large part of its activity is carried out in collaboration with different national and international research groups, supported by collaborative projects and by participation in national and international research networks.
The group has trained a large number of predoc and postdoc researchers. Most of them are currently active in some of the best international research institutions.

In addition to an accredited group in the cardiovascular research line of INCLIVA, it is a group recognized by the University of Valencia (GIUV2013-009).

Dr. Carlos Hermenegildo Caudevilla
Dr. Carlos Hermenegildo Caudevilla

Dr. Carlos Hermenegildo Caudevilla
Dra. Susana Novella del Campo

Leading, R4

Carlos Hermenegildo Caudevilla    
Susana Novella del Campo    

Established, R3
Recognised/Emerging, R2

Daniel Bernardo Pérez Cremades
Elena Monsalve Villalba

First Stage, R1

Cristina Rosales Ariza

Collaborating Researchers
Administrative assistant
FAM222A, part of the BET-Regulated basal endothelial transcriptome, is a novel determinant of endothelial biology and angiogenesis-brief report. Tzani A, Haemmig S, Cheng H, Perez-Cremades D, Heuschkel M, Jamaiyar A, Singh S, Aikawa M, Yu P, Wang T, Sun Y, Feinberg M, Plutzky J. Arteriosclerosis, Thrombosis, and Vascular Biology. 2024 Jan;44(1):143-155. doi: 10.1161/ATVBAHA.123.319909. PMID: 37942611

A miRNA/CXCR4 signaling axis impairs monopoiesis and angiogenesis in diabetic critical limb ischemia. Cheng H, Zhuang R, Perez-Cremades D, Chen J, Jamaiyar A, Wu W, Sausen G, Tzani A, Plutzky J, Henao-Mejia J, Goodney P, Creager M, Sabatine M, Bonaca M, Feinberg M. JCI Insight. 2023 Apr 10;8(7):e163360. doi: 10.1172/jci.insight.163360. PMID: 36821386

Cardiovascular complications of diabetes: role of non-coding RNAs in the crosstalk between immune and cardiovascular systems. Spinetti G, Mutoli M, Greco S, Riccio F, Ben-Aicha S, Kenneweg F, Jusic A, de Gonzalo-Calvo D, Nossent A, Novella S, Kararigas G, Thum T, Emanueli C, Devaux Y, Martelli F. Cardiovascular Diabetology. 2023 May 24;22(1):122. doi: 10.1186/s12933-023-01842-3. PMID: 37226245

Extracellular histones trigger oxidative stress-dependent induction of the NF-kB/CAM pathway via TLR4 in endothelial cells. Perez-Cremades D, Bueno-Beti C, Garcia-Gimenez J, Ibanez-Cabellos J, Pallardo F, Hermenegildo C, Novella S. Journal of Physiology and Biochemistry. 2023 May;79(2):251-260. doi: 10.1007/s13105-022-00935-z. PMID: 36464762

Impaired angiogenesis in diabetic critical limb ischemia is mediated by a miR-130b/INHBA signaling axis. Cheng H, Perez-Cremades D, Zhuang R, Jamaiyar A, Wu W, Chen J, Tzani A, Stone L, Plutzky J, Ryan T, Goodney P, Creager M, Sabatine M, Bonaca M, Feinberg M. JCI Insight. 2023 May 22;8(10):e163041. doi: 10.1172/jci.insight.163041. PMID: 37097749

MicroRNA-375 repression of Kruppel-like factor 5 improves angiogenesis in diabetic critical limb ischemia. McCoy MG, Jamaiyar A, Sausen G, Cheng HS, Perez-Cremades D, Zhuang R, Chen J, Goodney PP, Creager MA, Sabatine MS, Bonaca MP, Feinberg MW. Angiogenesis. 2023 Feb;26(1):107-127. doi: 10.1007/s10456-022-09856-3. PMID: 36074222

MicroRNA-mediated control of myocardial infarction in diabetes. Perez-Cremades D, Chen J, Assa C, Feinberg M. Trends in Cardiovascular Medicine. 2023 May;33(4):195-201. doi: 10.1016/j.tcm.2022.01.004. PMID: 35051592

A miRNA cassette reprograms smooth muscle cells into endothelial cells. McCoy MG, Perez Cremades D, Belkin N, Peng W, Zhang B, Chen J, Sachan M, Wara AKMK, Zhuang R, Cheng HS, Feinberg MW. FASEB Journal. 2022 Apr;36(4):e22239. doi: 10.1096/fj.202101872R. PMID: 35235229

Circulating miRNA fingerprint and endothelial function in myocardial infarction: comparison at acute event and one-year follow-up. Mompeon A, Perez-Cremades D, Paes A, Sanchis J, Ortega-Paz L, Andrea R, Brugaletta S, Sabate M, Novella S, Dantas A, Hermenegildo C. Cells. 2022 Jun 2;11(11):1823. doi: 10.3390/cells11111823. PMID: 35681518

Dysregulated genes, MicroRNAs, biological pathways, and gastrocnemius muscle fiber types associated with progression of peripheral artery disease: a preliminary análisis. Saini S, Perez-Cremades D, Cheng H, Kosmac K, Peterson C, Li L, Tian L, Dong G, Wu K, Bouverat B, Wohlgemuth S, Ryan T, Sufit R, Ferrucci L, McDermott M, Leeuwenburgh C, Feinberg M. Journal of the American Heart Association. 2022 Nov;11(21):e023085. doi: 10.1161/JAHA.121.023085. PMID: 36300658

Editorial: Genetic and gene regulation underlying sex differences in cardiovascular disease. Robinson E, Novella S. Frontiers in Cardiovascular Medicine. 2022 Apr 11;9:881154. doi: 10.3389/fcvm.2022.881154. PMID: 35479282

Endothelial cell-specific deletion of a microRNA accelerates aterosclerosis. Yang D, Haemmig S, Chen J, McCoy M, Cheng H, Zhou H, Cheng X, Sun X, Haneo-Mejia J, Vellarikkal S, Gupta R, Barrera V, Perez Cremades D, Feinberg M. Atherosclerosis. 2022 Jun;350:9-18. doi: 10.1016/j.atherosclerosis.2022.04.010. PMID: 35462240

Extracellular histones activate endothelial NLRP3 inflammasome and are associated with a severe sepsis phenotype. Beltrán-García J, Osca-Verdegal R, Pérez-Cremades D, Novella S, Hermenegildo C, Pallardó FV, García-Giménez JL. Journal of Inflammation Research. 2022 Jul 25;15:4217-4238. doi: 10.2147/JIR.S363693. PMID: 35915852

Histone citrullination mediates a protective role in endothelium and modulates inflammation. Osca-Verdegal R, Beltran-Garcia J, Paes A, Nacher-Sendra E, Novella S, Hermenegildo C, Carbonell N, Garcia-Gimenez J, Pallardo F. Cells. 2022 Dec 15;11(24):4070. doi: 10.3390/cells11244070. PMID: 36552833

Isolation and culture of murine aortic cells and RNA isolation of aortic intima and media: Rapid and optimized approaches for atherosclerosis research. Chen J, Zhuang R, Cheng HS, Jamaiyar A, Assa C, McCoy M, Rawal S, Perez-Cremades D, Feinberg MW. Atherosclerosis. 2022 Mar 11;347:39-46. doi: 10.1016/j.atherosclerosis.2022.03.011. PMID: 35306416

KLF10 deficiency in CD4+T cells promotes atherosclerosis progression by altering macrophage dynamics. Wara AK, Rawal S, Yang X, Perez-Cremades D, Sachan M, Chen J, Feinberg MW. Atherosclerosis. 2022 Oct;359:27-41. doi: 10.1016/j.atherosclerosis.2022.08.019. PMID: 36174463

miR-181b regulates vascular endothelial aging by modulating an MAP3K3 signaling pathway. Zhou H, Yang D, Cheng H, McCoy M, Perez-Cremades D, Haemmig S, Wong D, Chen L, Feinberg M. FASEB Journal. 2022 Jun;36(6):e22353. doi: 10.1096/fj.202200046R. PMID: 35593587

p Deficiency of lncRNA SNHG12 impairs ischemic limb neovascularization by altering an endothelial cell cycle pathway. Gross DA, Cheng HS, Zhuang R, McCoy MG, Perez-Cremades D, Salyers Z, Wara AKMK, Haemmig S, Ryan TE, Feinberg MW. JCI Insight. 2022 Jan 11;7(1):e150761. doi: 10.1172/jci.insight.150761. PMID: 34793334

Perivascular fibrosis is mediated by a KLF10-IL-9 signaling axis in CD4+ T Cells. Zhuang R, Chen J, Cheng HS, Assa C, Jamaiyar A, Pandey AK, Perez-Cremades D, Zhang B, Tzani A, Khyrul Wara A, Plutzky J, Barrera V, Bhetariya P, Mitchell RN, Liu Z, Feinberg MW. Circulation Research. 2022 May 27;130(11):1662-1681. doi: 10.1161/CIRCRESAHA.121.320420. PMID: 35440172

A smooth muscle cell-enriched long noncoding RNA regulates cell plasticity and atherosclerosis by interacting with serum response factor. Ni H, Haemmig S, Deng Y, Chen J, Simion V, Yang D, Sukhova G, Shvartz E, Wara A, Cheng H, Perez-Cremades D, Assa C, Sausen G, Zhuang R, Dai Q, Feinberg M. Arteriosclerosis, Thrombosis, and Vascular Biology. 2021 Sep;41(9):2399-2416. doi: 10.1161/ATVBAHA.120.315911. PMID: 34289702

Methotrexate attenuates vascular inflammation through an adenosine-microRNA-dependent pathway. Yang D, Haemmig S, Zhou H, Perez-Cremades D, Sun X, Chen L, Li J, Haneo-Mejia J, Yang T, Hollan I, Feinberg M. Elife. 2021 Jan 8;10:e58064. doi: 10.7554/eLife.58064. PMID: 33416495

MiR-409-3p targets a MAP4K3-ZEB1-PLGF signaling axis and controls brown adipose tissue angiogenesis and insulin resistance. Becker-Greene D, Li H, Perez-Cremades D, Wu W, Bestepe F, Ozdemir D, Niosi C, Aydogan C, Orgill D, Feinberg M, Icli B. Cellular and Molecular Life Sciences. 2021 Dec;78(23):7663-7679. doi: 10.1007/s00018-021-03960-1. PMID: 34698882

Regulatory network analysis in estradiol-treated human endothelial cells. Perez-Cremades D, Paes A, Vidal-Gomez X, Mompeon A, Hermenegildo C, Novella S. International Journal of Molecular Sciences. 2021 Jul 30;22(15):8193. doi: 10.3390/ijms22158193. PMID: 34360960

Reference: PI22/01083
Title: Mecanismos moleculares de las rutas de señalización de los miRNA modificados por infarto agudo de miocardio. Influencia del sexo y el envejecimiento.
Funding body: Instituto de Salud Carlos III – Cofinanciado FEDER
Principal Investigator: Suanna Novella del Campo, Carlos Hermenegildo Caudevilla
Duration: 2023 – 2025
Total budget: 111.320,00 €
Reference: CIGE/2021/158
Title: Caracterización de un modelo murino experimental de insuficiencia cardiaca para estudios transcriptómicos.
Funding body: Conselleria de Innovación, Universidades, Ciencia y Sociedad Digital
Principal Investigator: Daniel Pérez Cremades
Duration: 2022 – 2023
Total budget: 20.000 €
Reference: AICO/2021/211
Title: Análisis funcional de las rutas de señalización vascular reguladas por miRNA sensibles a estrógenos
Funding body: Conselleria de Innovación, Universidades, Ciencia y Sociedad Digital
Principal Investigator: Susana Novella del Campo, Carlos Hermenegildo Caudevilla
Duration: 2022 – 2024
Total budget: 90.000 €
Reference: COST Action CA21153
Title: Network for implementing multiomics approaches in atherosclerotic cardiovascular disease prevention and research (AtheroNET).
Funding body: European Commission
Principal Investigator: Paolo Magni
Duration: 2022 – 2025
Total budget: 0
Reference: PI19/01714
Title: Regulación dependiente de sexo y estrógenos en el síndrome coronario agudo e impacto funcional en  células endoteliales humanas. Nuevos biomarcadores para prevención primaria
Funding body: Instituto de Salud Carlos III – Cofinanciado FEDER
Principal Investigator: Carlos Hermenegildo Caudevilla, Susana Novella Del Campo
Duration: 2020 – 2023
Total budget: 159.720 €
Reference: AICO/2020/030
Title: Repercusión en la función vascular de miRNA regulados por estrógenos
Funding body: Conselleria de Innovación, Universidades, Ciencia y Sociedad Digital
Principal Investigator: Susana Novella del Campo
Duration: 2020 – 2021
Total budget: 40000
Reference: COST Action CA17129
Title: Catalysing transcriptomics research in cardiovascular disease
Funding body: European Commission
Principal Investigator: Yvan Devaux
Duration: 2018 – 2023
Total budget: 0
Reference: PI16/00229
Title: Perfil plasmático de miRNA en infarto agudo de miocardio: relación con la evolución clínica en pacientes y con la función cardiovascular y posible terapia con micropartículas en ratones
Funding body: Instituto de Salud Carlos III – Cofinanciado FEDER
Principal Investigator: Carlos Hermenegildo Caudevilla
Duration: 2017 – 2020
Total budget: 134.915 €
+ Info
Title: Análisis de la expresión de mirna circulantes en pacientes con infarto agudo de miocardio y su relación con citoquinas. Repercusión funcional en cultivos de células endoteliales humanas
Doctoral candidate: Mompeón Campos, Ana
Director(s): Hermenegildo Caudevilla, Carlos; Novella Del Campo, Susana
Date of the defense: 04/03/2019
University: Universitat de València